Retrospective analysis of the peripheral blood cellular composition in 57 persons diagnosed with leukemia (ICD-9, codes 204-208) was performed based on a 60-year follow-up of the Techa riverside residents affected by chronic radiation exposure. The analysis covered three periods: that of maximal radiation exposure (1950-1956), of hematopoiesis recovery (1957-1970), and of late effects (after 1970). Peripheral blood cellular composition in exposed persons was also studied during the latency period. People affected by chronic radiation exposure (mean absorbed dose to red bone marrow amounted to 0.77±0.21 Gy) and diagnosed with leukemia did not exhibit any peculiar features of cellular composition of blood during the period of maximal radiation exposure, hematopoiesis recovery, late effects, and also during the latency period of leukemia.

Patients with internal endometriosis (adenomyosis) at the time of being hospitalized were revealed an increase in concentration of lipid peroxidation products and reduction in antioxidatic protection factors at the systemic and local levels. The blood serum revealed an increase in α₂-macroglobuline, stable nitrogen oxide metabolites, and C-reactive protein and decrease in α₁-antitripsine level. The essential reduction of glycero- and sphingophospholipids, cholesterol ethers, mono- and diglycerols and increase in the level of lysophosphatidylcholine, free cholesterol, triacylglycerol, free fatty acids were determined in membrane of erythrocytes. Inefficiency of complex standard treatment in correction of the metabolic status disturbances was established.

The method for the complex evaluation of polymorbidity (MCEP) is designed to improve the treatment and diagnostic processes in the context of primary health care. Validation of the MCEP was carried out during a clinical study of patients with a metabolic syndrome against the background of polymorbidity. External and substantive validity was assessed using a survey of practicing physicians and experts. Reliability is confirmed by assessing internal consistency and reproducibility. Criterial validity is confirmed by the strong feedback of the indices of the MCEP and the results of the most common methodologies for its evaluation. The sensitivity of the MCEP was proved by calculating predictable differences in its indices. As a result of the validation of the MCEP, its external, substantial, constructive, and criterial validity, reliability and sensitivity are proved. The MCEP is recommended for use in medical organizations providing primary health care.

Cardiovascular diseases continue to occupy a leading position in the disability and mortality of the population. The most dangerous diseases associated with the fatal outcome in the structure of coronary heart disease remain its acute forms, primarily acute coronary syndrome (ACS), the outcome of which are acute myocardial infarction (AMI) and unstable angina (UA). Trigger role of hypersympathicotonia and the disturbance of the psycho-emotional state in the pathogenesis of these diseases have been proved. In this regard, the study of the psycho-emotional and functional state of patients entering the emergency cardiology department with a diagnosis of ACS, on the background of hospital and post-hospital rehabilitation is important and relevant. The article presents the results of a comparative assessment of the psycho-emotional and functional state of patients with AMI and UA.

We examined 296 patients with atrial fibrillation and arterial hypertension consisting of a collection of complaints, anamnesis, and objective examination, measurement of waist circumference (WS), the study of leptin levels in blood of the patients with BMI 25 kg/m² or more at the beginning of the observation, electrocardiographic examination (ECG), echocardiography at the beginning and at the end of the study. Paroxysms of atrial fibrillation were recorded when performing ECG in case of occurrence of symptoms typical for AF, but asymptomatic forms were detected by daily monitoring the regularity of the radial pulse with the subsequent ECG to confirm AF. A patient should be observed for a year. Overweight and abdominal obesity affect the course of atrial fibrillation, increasing the number of repeated paroxysms. Predictors of repeated paroxysms of atrial fibrillation were women with WS more than 80 cm, the hormone content above 8.7 mg/ml and diastolic blood pressure over 90 mmHg and men with BMI 25 kg/m² or more.

The article considers the features of the course of reactive arthritis in children. Etiological factors such as intestinal, urogenital and respiratory infections have been identified. Most often, reactive arthritis was diagnosed in pre-school girls. In more than 65% of cases, children have a history of acute respiratory infections, chronic foci of infection, such as tonsillitis, quinsy, and caries. A third of the children surveyed had a family history of rheumatic fever. It was found that in most patients the disease had acute character. Among the clinical manifestations the most common lesions of the hip and knee joints were monoarticular type, in all patients in the onset of the disease the joint syndrome was characterized by moderate edema of the joint.

Benign prostatic hyperplasia (BPH) is one of the most common diseases in men of elderly and senile age. Currently, medical therapy for BPH takes priority, but surgical treatment has not lost its relevance, and its frequency remains stable for 10 years. The review presents an analysis of publications highlighting the problem of large-scale BPH surgery, the causes and sources of intraoperative blood loss and techniques aimed at minimizing them. At the stages of retropubic adenomectomy, the authors propose different modifications to the prevention of hemorrhagic complications. The presented articles indicate differences in views on the anatomy of the plexus Sartolini and the proposed methods of surgical hemostasis. The need for further study of the surgical anatomy of the vascular architectonics of the prostate gland as an important factor in the reduction of perioperative complications and lethality is indicated.

The article presents the results of investigating the self-service skills formation in the program of restorative education for young post-stroke patients. Comparative analysis of the praxis functions in post-stroke young patients before and after rehabilitation training made it possible to reveal a significant increase in the rate and accuracy of the kinetic, kinesthetic, and spatiotemporal praxis; increasing the differentiation and coordination of the seriated organization of movements and actions. In post-stroke young patients with a lesion of the non-leading (right) cerebral hemisphere, a more intensive dynamics is observed both in restoring the praxis functions (estimated according to the criteria of pace, accuracy, differentiation and coordination), and in developing small self-service skills in comparison with patients with the lesion in the dominant (left) hemisphere. The intensity of functional restoration with the subsequent formation of the skill is due to the involvement of the non-leading hemisphere and is interrelated with an increase in the accuracy and pace of motor functions.

On analyzing the results of managing patients with hepatic cirrhosis, who underwent portocaval anastomoses from 2003 to 2017, a therapeutic and diagnostic algorithm for preventing early postoperative thrombosis of decompressive venous anastomoses was developed by using a combination of pharmacological and mechanical preventive methods. Improving the technique of surgeries, anticoagulant prevention, which began in the preoperative period and continued after portocaval bypass surgery, avoids thrombotic complications in patients. Dynamic control of the patency of portocaval anastomosis allows to detect early signs of the impaired shunt patency and to take measures for its recanalization. Daily monitoring and evaluation of changes in hemostasiological parameters allows you to identify the severity of thrombinemia and monitor anticoagulant therapy.

The purpose of the study was to determine the contribution of RANKL/OPG transmembrane molecule system into the development of pathological arterial stiffness in rheumatoid arthritis (RA). RA patients (n=181) were randomized into 4 groups based on RF/ACCP (rheumatoid factor/antibodies to cyclic citrullinated peptide) positivity and disease duration (less than or more than 2 years). Serum osteoprotegerin (OPG) and soluble receptor activator of NF-kB ligand (sRANKL) concentrations, as well as pulse wave contour analysis were evaluated in all patients. The largest OPG level was detected in Group 1 patients; sRANKL concentration with maximal RANKL/OPG ratio was predominant in Group 4 patients. Prolonged course of RF/ACCP-seropositive RA was characterized by more significant arterial rigidity shifts (increases in augmentation (AIp), stiffness (SI), reflection (RI) indices). Correlation analysis revealed the presence of significant relations between the RANKL/OPG transmembrane molecule system and arterial rigidity parameters in RA.

The experiment studied the content and ratio of proteins and lipids in erythrocyte membrane on the model of acute destructive pancreatitis associated with 5-, 30-and 60-day alcohol intoxication. Under the conditions of pancreatitis, largely against the background of alcohol- associated pancreatitis, the changes in the content of proteins responsible for structurization and stabilization of erythrocyte membrane (α-and β-spectrin, dematin, ankyrin, band 4.1 protein, pallidin), shaping and flexibility (actin, tropomiozin), and intracellular erythrocyte metabolism (anion-transport protein, glyceraldehyde-3-phosphate dehydrogenase, glutathione-S-transferase, band 4.5 protein) were revealed. The detected disturbances of the content and ratio of the lipid fractions playing the principle role in sequencing proteinaceous macromolecules and erythrocyte metabolism along with the changes in ordonnance of proteins break the intracellular metabolism and functional properties of erythrocytes.

Minimal inhibitory concentrations (MICs) of 17 officinal aqueous medicinal herbal extracts in regard to antibiotic-sensitive and extensively antibiotic-resistant P. aeruginosa and A. baumannii isolates were determined. The expressed antibacterial activity (MIC < 1 mg/mL) was found for aqueous cowberry ( Vaccinium vitis-idaea ), eucalyptus ( Eucalyptus viminalis ), oak ( Quercus robur ), and bearberry ( Arctostaphylos uva-ursi ) extracts. Aqueous herbal extracts did not influence significantly the antibacterial activity of aminoglycosides, carbapenems and fluoroquinolones during their combined action on a microbial cell. A universal dose-dependent antagonistic effect of herbal extracts on the colistin microbiological efficacy in relation to A. baumannii and P. aeruginosa was revealed. A synergistic antibacterial effect (SFIC 0.25-0.50) of the combination of aqueous eucalyptus extract with the ceftazidime against 29.2% of OXA-carbapenemase producing A. baumannii strains was detected.

The article presents the results of experimental study of the light strengthened endoprosthesis on rabbits of chinchilla specie at onlay and sublay implantation in the abdominal wall tissues. There was done morphological study of abdominal tissue samples with implanted material. The area of cellular infiltrate located around the threads of endoprosthesis in cellular layer of the capsule, and area of newly formed connective tissue capsule were investigated. Cellular index was calculated as the ratio of the histiocytic cells to the inflammatory infiltrate cells. In the result of the study authors prove that at early periods the more rapid stopping of inflammatory changes and formation of the connective tissue capsule take place around endoprosthesis at sublay implanting, while at later periods reparative response does not depend on arrangement option of prosthesis.

The study was performed on adult skulls systematized by the occlusion type and the degree of lower dentition preservation. It has been established that there are no differences in the structure of the lower jaw head between skulls with transitional forms of normal occlusion and orthognathic occlusion, but for different types of anomalous occlusion there are statistically significant differences in the main morphometric parameters of the condylar process. It has been established that complete teeth loss significantly changes the sagittal diameter of the mandible head. Therefore, the absence of teeth makes the lower jaw head conical-shaped in most cases (in 61.3%). The features of the mandibular head structure in the dentoalveolar conditions studied can cause changes in the temporomandibular joint, and, accordingly, lead to its dysfunctional disorders.

The survey analyzes the literature data on biosynthesis, reception, degradation, and physiological effects of the melanocortin family regulatory peptides. Information on the interaction of melanocortins with neurotransmitters of the brain, as well as their effect on the functional state of the organism regulatory systems, is presented. The neurotropic and immunotropic effects of peptides of the melanocortin family are reflected, as well as their effect on separate organs and systems, in particular: liver, cardiovascular system and hemostasis. The main pharmacological effects and mechanisms of action of the "Semax" drug created on the basis of the ACTH synthetic fragment are described. The data presented in the survey reflect the significant physiological activity and polyfunctionality of regulatory peptides of the melanocortin family, substantiated by a considerable number of studies, as well as the prospects of creating new pharmacological melanocortin-based preparations.

The influence of a non-selective arginase inhibitor - L-norvaline and its combination with cytoflavin, kudesan, hypoxenum on the functional activity of neutrophils, lipid peroxidation intensity and concentration of stable nitrogen metabolites in rat blood serum under interval histotoxic hypoxia has been studied. It has been determined that the administration of L-norvaline and antihypoxic drugs with different action mechanisms increases the metabolic activity of neutrophils, increases to a various degree neutrophils phagocytic activity, enhances the total antioxidant activity and reduces the content of lipid peroxidation secondary products. The introduction of L - norvaline, cytoflavin and hypoxenum most markedly affects the concentration of stable NO metabolites in the serum of rats, increasing this index to the level of stated values.

Polymorphic genes encoding matrix metalloproteinases contribute to the development and outcome of cerebrovascular diseases. The purpose of this study was to analyze the association between polymorphisms of matrix metalloproteinases genes MMP10 (rs486055) and MMP12 (rs2276109) with the risk of ischemic stroke (IS). We examined 1,073 unrelated Russian individuals from Central Russia including 666 IS patients and 407 controls. Genotyping of the MMP10 and MMP12 gene polymorphisms was carried out by TaqMan-based polymerase chain reaction. We found the association of genotype rs486055-C/T of MMP10 with an increased risk of ischemic stroke in smokers (OR = 1.56 95% CI 1.02-2.39, P = 0.03), while non-smoker carriers of the genotype did not possess disease risk. The present study was the first to show the association of genotype rs486055-C/T of the MMP10 gene with an increased risk of ischemic stroke in cigarette smokers.

Special features of isolating ethyl acetate of 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane (bensultap) from its artificial mixtures with the putrefied liver tissue upon infusion mode have been investigated. Double infusion of biological matrix with ethyl acetate at a mass ratio of isolation liquid to biologic object 2:1 and the 30 minutes running time of each infusion stage were necessary to isolate 75.39-75.42 % of 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane (bensultap) from putrescent liver. Purification of the isolated substance from endogenous compounds has been carried out by the method of micro-column chromatography (stationary phase - silica gel L 40/100 μm, eluents - hexane and a mixture of hexane-dioxane-propanol-2 (8: 3: 0.6)). The procedure for determining 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane (bensultap) in the putrefied liver tissue f by using normal-phase HLPC variant has been worked out. The procedure proposed has been validated by criteria of linearity, trueness, precision, and stability. Detection limit and quantitation limit for 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane were correspondently 3.15 10^-6 g and 4.0 10^-6 g per 1 g of biomaterial.

The possibility of ceftriaxone determination by IR spectroscopy in biological material is studied, as well as the possibility of proving the authenticity of a functional sample of the antibiotic analyzed in comparison with a standard sample. The studies were carried out on the prototyping mixtures of the analyte with the tissue of the cadaveric liver, which had no signs of a putrefactive change. The IR spectra of the functional and reference samples were found to coincide with characteristic absorption bands that prove the possibility of using a functional sample for further research with a view to economic benefit. The possibility of applying IR spectroscopy for the identification of ceftriaxone in extracts from biological material is substantiated. The practice of isolation and purification of ceftriaxone extracted from biological material has been applied in practice.