Effectiveness of proteins of allogeneic hepatocytes in the correction of immunometabolic disorders in acute ischemic liver damage

Objective. The aim of the study was to establish the effectiveness of the culture fluid of allogeneic hepatocytes (CFAH) and proteins isolated from them in correcting metabolic and immune disorders in acute ischemic liver damage. Material and methods. The study has been conducted on 105 healthy mature Wistar rats. Acute ischemic liver damage (AILD) was induced by cross clamping of the hepatoduodenal ligament for 20 minutes. Isolated allogeneic hepatocytes from newborn animals were used to prepare a culture fluid from which proteins were isolated. The culture fluid of allogeneic hepatocytes (CFAH) and their proteins obtained were administered with the start of modeling acute liver ischemia five times (with a 24-hour interval) intraperitoneally to rats with AILD at the rate of 5 mg/kg. Results. Acute liver ischemia causes the development of biochemical syndromes of the liver damage (cytolysis, intra- and extrahepatic cholestasis, toxic damage, insufficiency of synthetic processes) activates the processes of lipid peroxidation, disturbs intra-erythrocyte metabolism, and causes the development of oxidative stress and suppression of forming the adaptive and innate immunity. Introduction of CFAH, undivided CFAH proteins or CFAH proteins with molecular mass (MM) less than 130 kDa to animals with AILD normalizes or corrects the studied biochemical and immunological parameters. CFAH proteins with MM more than 130 kDa do not have such effects. Conclusion. During the analysis of CFAH or CFAH proteins corrective activity on the dynamics of biochemical markers of AILD, formation of humoral and cellular immunity, impaired functional and metabolic activity of peripheral blood neutrophils, intraerythrocyte metabolism, activation of free radical oxidation, the following sequence has been established (according to increasing the effectiveness): the lack of this activity in CFAH proteins with MM more than 130 kDa; the presence of normalizing and correcting activity of: CFAH - CFAH undivided proteins - CFAH proteins less than 130 kDa.

иммунометаболические нарушения, белки культуральной жидкости аллогенных гепатоцитов, остря ишемия печени, immunometabolic disorders, culture proteins of allogeneic hepatocytes, acute liver ischemia