Development and validation of procedure for determining 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane in putrefied liver tissue
https://doi.org/10.21626/vestnik/2018-1/18
Abstract
Special features of isolating ethyl acetate of 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane (bensultap) from its artificial mixtures with the putrefied liver tissue upon infusion mode have been investigated. Double infusion of biological matrix with ethyl acetate at a mass ratio of isolation liquid to biologic object 2:1 and the 30 minutes running time of each infusion stage were necessary to isolate 75.39-75.42 % of 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane (bensultap) from putrescent liver. Purification of the isolated substance from endogenous compounds has been carried out by the method of micro-column chromatography (stationary phase - silica gel L 40/100 μm, eluents - hexane and a mixture of hexane-dioxane-propanol-2 (8: 3: 0.6)). The procedure for determining 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane (bensultap) in the putrefied liver tissue f by using normal-phase HLPC variant has been worked out. The procedure proposed has been validated by criteria of linearity, trueness, precision, and stability. Detection limit and quantitation limit for 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane were correspondently 3.15 10-6 g and 4.0 10-6 g per 1 g of biomaterial.
About the Authors
Yu. N. Baranov
Kursk State Medical University
Russian Federation
Russian Federation
V. K. Shormanov
Kursk State Medical University
Russian Federation
Russian Federation
A. V. Nesterova
Kursk State Medical University
Russian Federation
Russian Federation
E. A. Kovalenko
Kursk State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Baranov Yu.N., Shormanov V.K., Nesterova A.V., Kovalenko E.A. Development and validation of procedure for determining 2-dimethylamino-1.3-bis-(phenylsulfonylthio) propane in putrefied liver tissue. Kursk Scientific and Practical Bulletin "Man and His Health". 2018;(1):121-127. (In Russ.) https://doi.org/10.21626/vestnik/2018-1/18
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