Identification of 2-acetyloxybenzoic acid in blood plasma
Abstract
The features of the 2-acetylhydroxybenzoic acid identification in blood plasma have been studied. An acetone has been suggested as an isolating agent for extraction of 2-acetylhydroxybenzoic acid from biological material. The possibility of purifying the analyte from endogenous substances by the adsorptive chromatography in a silica gel "L 40/100 microns" column using a mobile phase of hexane and diethyl ether (5:5) has been shown. To identify and quantify 2-acetylhydroxybenzoic acid in extractions from biomaterials we have suggested the methods of thin-layer chromatography of silica gel, ultraviolet and infrared spectrophotometry and GLC combined with mass selective detection. The technique of chemical and toxicological studies of 2-acetylhydroxybenzoic acid in blood plasma, which allows to identify 88.19-89.23 ± 2.29-3.56% of the compound with its proportion of 2.5-50.0 mg in 25g biofluid, has been developed.
About the Authors
V. V. Shormanov
Kursk State Medical University, Kursk
Russian Federation
Russian Federation
V. V. Chupak
Orel State University, Orel
Russian Federation
Russian Federation
E. O. Salykina
Kursk State Medical University, Kursk
Russian Federation
Russian Federation
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Review
For citations:
Shormanov V.V., Chupak V.V., Salykina E.O. Identification of 2-acetyloxybenzoic acid in blood plasma. Kursk Scientific and Practical Bulletin "Man and His Health". 2015;(1):109-114. (In Russ.)
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