Evaluation of the anti-inflammatory effect of dry red-stem buckwheat extract in a model experiment on rats

Objective - study the anti-inflammatory activity of a 10% mild dosage form for topical application of red-stem buckwheat on a model of formalin edema of the hind paw of rats.

Materials and methods. The object of the study was a dry extract of red-stem buckwheat. Confirmation of the presence of flavonoids in the dry extract was carried out by thin-layer chromatography. Quantitative determination of the total content of flavonoids, in terms of rutin, was carried out by differential spectrophotometry. The anti-inflammatory activity of red-stem buckwheat extract was studied using the "acute formalin edema" model in white Wistar rats.

Results. According to the results of the study, it was proved that the dry extract contains flavonoids (rutin, quercetin, isoquercetin), the total content was 8.03%. When buckwheat extract was applied topically as a 10% gel to the affected area for 3 days after modeling formalin edema of the rat's hind paw, it had a pronounced decrease in inflammatory edema by 70.37% (p<0.05), comparable to the effect of 5% diclofenac sodium gel 74.07% (p<0.05) and surpasses the effect of dimexide gel by more by more than 30% (p<0.05).

Conclusion. The data obtained as a result of the experiments indicate the prospects for further development of dosage forms with red-stem buckwheat extract.

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