Persistence of flutamide in biological material
Abstract
The flutamide persistence in decomposing organic matter (liver tissue) at three temperature regimes of conservation: 18-22оС, 8-10оС, and 1.5-2оС was investigated. The substance under test was isolated from the model mixtures with biological matter by infusing with acetone for 30 minutes twice. The purification of the extracted substance was carried out in a silica gel column L 40/100 mkm, eluting hexane-acetone with a mixed solvent (8:2). The optimum conditions for the identification and quantification of flutamide extracted from putrid liver tissue by TLC, GC-MS and spectrophotometry in the UV and visible regions of the spectrum. It is found that the increase in temperature from 2°C to 22°C reduces the duration og flutamide persistence from 32 to 52 days.
About the Authors
V. K. Shormanov
Kursk State Medical University
Russian Federation
Russian Federation
Yu. V. Andreyeva
Kursk State Medical University
Russian Federation
Russian Federation
V. A. Omelchenko
Forensic Center of Home Office Department of Orel Region
Russian Federation
Russian Federation
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Review
For citations:
Shormanov V.K., Andreyeva Yu.V., Omelchenko V.A. Persistence of flutamide in biological material. Kursk Scientific and Practical Bulletin "Man and His Health". 2016;(1):113-116. (In Russ.)
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