The results of the comparative amino acid analysis of species of hedysarum growing in the North Caucasus

The results of the complex study of the qualitative and quantitative composition of the aminoacids of the grass of three species of the genus Hedysarum growing in the territory of the North Caucasus are presented in the article. 

The objective of the work is to prepare the comparative aminoacid analysis in three samples of a species of Hedysarum growing in the territory of the North Caucasus. 

Materials and methods. Qualitative analysis of the amino acid composition was carried out by reaction with ninhydrin, and the quantification of free forms of amino acids was determined by photometric detection at a wavelength of 570 nm on an amino acid analyzer ААА-400. The content of free and bound amino acids was determined after staining the derivatives with ninhydrin and fixing their content at a wavelength of 440 and 570 nm. Analysis of the amino acid composition of these species Hedysarum daghestanicum, Hedysarum caucasicum, Hedysarum grandiflorum is given for the first time. 

Results. The comparative amino acid composition of the three studied specimens of the Hedysarum genus species growing on the territory of the North Caucasus showed that significant amino acids in the aboveground organs of the studied species were found, such as aspartic and glutamic acids, as well as proline, leucine, and phenylalanine. 

Conclusion. In conclusion, it should be noted that the bulk of the detected amino acids belongs to the group of essential amino acids, and in addition, the presence of proline and phenylalanine proves the presence of xanthones. The results of the study can be further used in the preparation of a comprehensive metabolic assessment of medicinal plant materials of species of the genus Hedysarum L.

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