Proof of poisoning with tropicamide introduced into stomach of warm-bloodedanimals, based on the detection of the original toxic agents and the compounds with the similar structure in organs and blood

Abstract

The possibility of poisoning with tropicamide after its oral introduction to warm-blooded animals has been studied in terms of the detection of the toxic agent and compounds with the similar structure (products of transformation) in poisoned organisms. After introducing LD50 of tropicamide intragastrically to warm-blooded animals (rats) in various intervals we were searching for the toxic agent and possible products of its transformation in various organs and blood of poisoned animals. The presence of tropicamide in all the examined organs and blood of poisoned animals was established. There we determined and identified compounds with the similar structure, such as N-ethyl-N-(pyridin-4-yl-methyl)-phenilacetamide, N-ethyl-2-phenyl-N-(pyridin-4-yl-methyl)-propenamid and N-ethyl-3-acetoxy-2-phenyl-N- (pyridin-4-yl-methyl)-propanamide in liver, stomach tissue and its contents, and in small intestine tissue. The possibility of poisoning by tropicamide as evidenced by detection in poisoned organisms of the toxic agent and three products of transformation with the similar structures was grounded.