Use of second-order spectra derivatives for estimation of mebendazole content in tablets

Abstract

Mebendazole (methyl 5-benzoyl-2-benzimidazole-carbamate) and Mebendazole tablets containing 0.1 g of effective agent were the subjects of the study. The character of absorbing electromagnetic radiation by the analyte under examination in a medium of dimethylsulfoxide in a wavelength range of 245-380 nm was investigated. The second-order derivatives of the peak absorption coefficient with 320 nm were determined. The presence of a linear dependence of the second-order derivatives of the specific absorption coefficient of Mebendazole electronic spectrum in dimethylsulfoxide medium on the analyte content in the photometrized solution (concentration range 1-20 μg / ml) was revealed. Based on the results of the data obtained, a calibration graph is constructed, its equation is calculated. The correlation coefficient exceeds 0.99. A relatively simple and easily feasible method for estimating the quantitative content of Mebendazole in tablets has been developed, which meets the criteria of linearity, selectivity, and necessary sensitivity.