Tissue engineered constructs for regenerative medicine based on deciduous dental pulp mesenchymal cells and new-generation polymer matrices

The aim of the present work was to develop an approach for producing tissue engineered constructs based on mesenchymal stromal cell culture and new-generation biodegradable synthetic polymer matrices obtained by the technology of surface-selective laser sintering. Primary mesenchymal stromal cells were derived from the pulp of naturally shed deciduous teeth. Following seeding, the cells were cultured on matrices for ten days. Cell viability was quantitatively assessed using CytoTox Assay (Promega) based on the colorimetric measurement of mitochondrial dehydrogenase activity. Cellular structure and spatial arrangement within a tissue-engineered matrix was visualized by scanning electron microscopy (SEM), using supravital lanthanoid staining. The results obtained showed that matrices under development enable good cell adhesion and proliferation, and hence can be used as a cell carrier in tissue engineering. Thus, the techniques of surface-selective laser sintering, which was developed by us, could find wide use in regenerative medicine.

поверхностно-селективное лазерное спекание, сканирующая электронная микроскопия, лантаноиды, мезенхимальные стволовые клетки, тканевая инженерия, urface-selective laser sintering, scanning electron microscopy, lanthanoids, mesenchymal stromal cells, tissue engineering

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