Features of the histological structure, chemical and elemental composition of the regenerate formed in the tibia after prolonged exposure to tartrazine and correction with sodium selenite

Objective - to study the histological structure, chemical and elemental composition of the tibial regenerate after 60-day exposure to tartrazine at different times of bone healing, as well as to establish the possible effectiveness of sodium selenite. Materials and methods. The experiment was carried out on ninety mature white male rats. For 60 days, rats were daily intragastrically injected with 1 ml of saline solution (Fiz+Def group) or a similar volume of tartrazine solution at a dose of 750 mg/kg in isolation (T750+Def group) or combined with intramuscular administration of selenase (T750Sel+Def group). On the day 61 of the experiment, a tibial fracture was simulated by applying a through defect. The histological structure of bone regenerate was studied by light microscopy and morphometry. The content of water, organic, and mineral substances in the regenerate was determined by the weight method. The content of calcium, magnesium, and zinc in bone ash was determined by atomic absorption spectrometry. Results. In the T750+Def group, the most pronounced changes relative to the Fiz+Def group were recorded on the 15th and 24th days of the experiment. The area of the regenerate granulation tissue was higher by 118,06% and 84,89%, the area of woven bone, lamellar bone was lower by 28,79% and 24,13%. The water content was higher by 4,50% and 12,91%, the organic matter content - by 7,00% and 7,49%, calcium content - by 6,67% and 5,89%, zinc content - by 16,04% and 10,56%. In the T750Sel+Def group, the parameter values rose to the control values faster than in the T750+Def group. An increase in the area of woven and lamellar bones was found by 23,37% and 24,90% on 24th day, the content of calcium and zinc - by 7,14%, 10,13% and 5,35%, 10,35% on 15th and 24th days. Conclusion. Sixty-day exposure to tartrazine at a dose of 750 mg/kg changes the histological structure, chemical, and elemental composition of the regenerate at different times of its formation, and the introduction of selenase with it reduces their severity.

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