Determination of metoprolol in biological material
https://doi.org/10.21626/vestnik/2025-1/09
EDN: RVYSLM
Abstract
Metoprolol belongs to beta-adrenoblockers, which are one of the most important classes of cardiovascular drugs, and is considered the basis of therapy in heart diseases such as heart failure and atrial fibrillation.
Оbjective - to develop a method for the determination of metoprolol in biological material.
Materials and methods. The object of the study is metoprolol ((±)-1- [4- (2-Methoxyethyl)phenoxy]-3- (1-methoxyethyl)amino]-2-propanol succinate). The model biological matrices were liver tissue and blood plasma.
Results. To isolate metoprolol from biological material the insistence in two stages with three times by weight amount of chloroform at half an hour duration of each contact of biomatrix and extractant was proposed. The possibility of purification of the analyzed compound isolated from biological objects by extraction and TLC methods was determined. Sufficient efficiency of the proposed purification scheme was shown.
Conclusion. The technique of identification and quantitative determination of metoprolol in extracts from cadaveric material using TLC and UV-spectrophotometry methods has been developed.
About the Authors
Lekso L. Kvachakhiya
Kursk State Medical University (KSMU)
Russian Federation
Russian Federation
Vladimir K. Shormanov
Kursk State Medical University (KSMU)
Russian Federation
Russian Federation
Alina P. Tyurina
Anastasia A. Abelyasheva
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Review
For citations:
Kvachakhiya L.L., Shormanov V.K., Tyurina A.P., Abelyasheva A.A. Determination of metoprolol in biological material. Humans and their health. 2025;28(1):67-72. (In Russ.) https://doi.org/10.21626/vestnik/2025-1/09. EDN: RVYSLM
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