Study of the antioxidant activity of the tuberous pea herb (Lathyrus tuberosus L.)

Objective: to study the antioxidant and antiradical activity of extracts from the tuberous pea herb. Materials and methods. The study object was tuberous pea herb collected in Kursk region in 2020-2021 during the flowering period of plants. The antioxidant and antiradical activities of aqueous and aqueous-alcoholic extracts from the herb of tuberous pea were investigated. Antioxidant activity was evaluated by titrimetric method based on the interaction of potassium permanganate and reducing substances contained in the extract from raw materials of tuberous pea herb. The antiradical activity was studied by spectrophotometric method according to the ability of extracts to inactivate 1,1-diphenyl-2-picrylhydrazyl. The content of phenolic compounds (direct spectrophotometry) in terms of chlorogenic acid and flavonoids (differential spectrophotometry) in terms of rutin was determined. The obtained data were processed by statistical analysis. Results. By the example of the use of different extractants: water and alcohol of different concentrations, we investigated their influence on the antioxidant and antiradical activity of tuberous pea herb. The analysis of the experimental data obtained showed that all the extracts studied have antioxidant and antiradical activity, with the maximum activity found in extracts obtained from the herb using 50% and 70% ethyl alcohol as an extractant. The maximum content of phenolic compounds and flavonoids was also found in the mentioned extracts, which indicates a correlation between the antioxidant activity and the content of phenolic compounds. Conclusion. The results of the conducted experimental study allow us to conclude that the tuberous pea herb harvested in the flowering phase can be considered as a promising medicinal plant raw material of antioxidant orientation.

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