The effect of alcohol abuse on the risk of acute non-biliary pancreatitis in carriers of the polymorphism rs7674870 gene SLC7A11 A>G

The aim was to determine the contribution of the rs7674870 single nucleotide polymorphism of the SLC7A11 A>G gene and alcohol abuse to the risk of acute pancreatitis.

Materials and methods. The material for the study was DNA samples obtained from 469 unrelated patients with acute non-biliary pancreatitis and 572 unrelated individuals without gastrointestinal diseases. The average age of the patients was 48.9 ± 13.1, healthy individuals - 47.8 ± 12.1. The diagnosis and severity of ANP were based on clinical symptoms, laboratory and instrumental methods of investigation. The study participants were divided into two groups depending on the amount of alcohol consumed per week: (1) - less than 200 g per week and (2) - more than 200 g per week; according to frequency: (1) - 1 to 2 days a month or less and (2) - 1 or more days a week; and duration: (1) - up to 10 years and (2) - for 10 or more years. Genomic DNA was purified from the thawed blood samples by phenol chloroform extraction method. Genotyping was performed by real-time PCR by discriminating alleles using TaqMan probes on a CFX96 Bio-Rad Laboratories amplifier (USA) using commercial TaqMan SNP Genotyping Assays reagent kits from Applied Biosystems (USA). Associations of alleles and genotypes with a risk of ANP were evaluated by the odds ratio (OR). Statistical analysis was performed with Statistica 10 (StatSoft, USA), SNPstats.

Results. We did not find any association of the rs7674870 single nucleotide polymorphism of the SLC7A11 A/G gene with the risk of ANP. However, the genotype-environment analysis revealed an association between the G/G SLC7A11 A/G rs7674870 genotype with a reduced risk of ANP in the absence of alcohol abuse in terms of frequency (OR=0.54, 95% CI=0.31-0.96, Р=0.02), duration (OR=0.66, 95% CI=0.44-0.99, Р=0.03), and amount of alcohol consumed per week (OR=0.63, 95% CI=0.41-0.97, Р=0.01).

Conclusion. The results obtained may indicate that, in carriers of the G/G SLC7A11 A/G rs7674870 genotype, moderate consumption of alcohol compensates for pathological changes that can lead to the development of ANP.

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