Influence of ACTH-4-7-Pro-Gly-Pro intraperitoneal injection on hepatocyte morpho-functional state under acute immobilization stress
Abstract
The aim of this study was to investigate the influence of semax, a synthetic analogue of ACTH (ACTH-4-7-Pro-Gly-Pro), on the morpho-functional condition of liver under chronic immobilization stress. The peptide was administered by intraperitoneal injection to male Wister rats at doses of 5, 50, 150, and 450 mcg/kg of body weight, 15 minutes before exposure to stress. Stress was performed by 4 h immobilization. Biochemical and morphometric examination showed that stress exposure significantly decreased the serum protein concentration, increased the activity of serum ALT and AST, and induced liver hydropic degeneration in the control group. Application of the peptide at the dose of 5, 50, and 150 mcg/kg resulted in improvement of the hepatocytes condition in central and peripheral parts of the lobules, but residual portions of hydropic degeneration remained in the intermediate sections. The further increase in the peptide dose up to 450 mcg/kg was characterized by residual hydropic degeneration in the central sections of liver lobules. Beside the manifestations mentioned, the peptide at the dose of 5 and 50 mcg/kg decreased the ALT activity, but increased it at the dose of 450 mcg/kg. The AST activity was reliably increased at the dose of 5 mcg/kg.
Keywords
гепатоциты,
стресс,
аналог АКТГ,
семакс,
печень,
hepatocytes,
stress,
analogue of ACTH-4-7-Pro-Gly-Pro,
semax,
liver
About the Authors
A. V. Ivanov
Kursk State Medical University
Russian Federation
Russian Federation
I. I. Bobyntsev
Kursk State Medical University
Russian Federation
Russian Federation
O. M. Shepeleva
Kursk State Medical University
Russian Federation
Russian Federation
A. A. Kryukov
Kursk State Medical University
Russian Federation
Russian Federation
N. F. Myasoedov
Institute of Molecular Genetics of Russian Academy of Sciences
Russian Federation
Russian Federation
L. A. Andreeva
Institute of Molecular Genetics of Russian Academy of Sciences
Russian Federation
Russian Federation
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Review
For citations:
Ivanov A.V., Bobyntsev I.I., Shepeleva O.M., Kryukov A.A., Myasoedov N.F., Andreeva L.A. Influence of ACTH-4-7-Pro-Gly-Pro intraperitoneal injection on hepatocyte morpho-functional state under acute immobilization stress. Kursk Scientific and Practical Bulletin "Man and His Health". 2015;(4):86-92. (In Russ.)
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