Assessment of the effect of hemostatic materials on blood clot formation in an in vitro experiment

Objective: to evaluate the time change in blood clotting while using different application hemostatic materials in an in vitro experiment. 

Materials and methods. As materials of the research we used the following samples of haemostatic materials: control group (group No. 1), Tachocomb (No. 2), Gelita-Spon Standard (No. 3), Surgicel Fibrillar (No. 4), the samples of hemostatic sponges developed together with "Linteks LLC" (St. Petersburg, Russia) on a basis of sodium- carboxymethyl cellulose (Na-CMC) with various modifications: Na-CMC+ Tranexamic acid, pressed (No. 5), Na-CMC+ Tranexamic acid, not pressed (No. 6), Na-CMC pressed (No. 7), Na-CMC not pressed (No. 8). The time assessment of coagulation was carried out according to the technique developed by us. 

Results. During the research it was established that all groups of tested samples accelerated the process of blood clot formation to different degree that was confirmed by statistically significant differences (p≤0.05). The most effective samples were those of group N 6, which accelerated the clotting time 1.73 times as compared to the control group (p≤0.05). Group N 8 samples accelerated clot formation time by 14.1 seconds, indicating that they had the least efficacy of the test samples. 

Conclusion. The samples developed by the authors are not inferior in efficiency to the foreign analogues used in clinical practice, such as Tachocomb, Gelatina-Spon Standart, Surgicel Fibrillar. The most effectiveness was shown by the modified implants of group No. 6 (Na-CMC Tranexamic acid, unpressed). This combined agent forms a blood clot quite rapidly, which indicates the need for further comprehensive examination and, in the long run, active use of this implant in surgical practice to stop bleeding.