Effect of chitosan-collagen complex with silver nanoparticles on the wound progress in experiment

Objective: to study the local effects of the chitosan-collagen complex with silver nanoparticles on the wound proress, as well as its hepato- and nephrotoxic effects. Materials and methods. For the study, we used dressing samples based on high-molecular polymers, which were a highly porous sponge made of chitosan-collagen polyelectrolyte complex with the inclusion of colloidal (ultrafine) particles of metallic (zero-valent) silver. The experiment was performed on white Wistar rats. Evaluation of the results was carried out on the basis of planimetric, bacteriological, histological, biochemical, and hematological methods. The results are statistically processed. Results. It was revealed that by the 15^th day the wound area had decreased by 96% of the original size, the maximum healing rate was noted on 1-5 days of treatment, and no significant changes were found in hematological and biochemical blood tests. Histological examination of wound microscopic specimens revealed that by the 15th day the peak of the proliferative phase of inflammation had occurred, the data were also confirmed on the basis of a morphometric study and a hydroxyproline test. Conclusion. The study revealed that the use of chitosan-collagen complex with silver nanoparticles has a positive effect on the course of the wound process by reducing the microbial contamination of the wound, stimulating tissue regeneration, with no negative effect on the liver and kidneys.

wound treatment, chitosan-collagen complex, silver nanoparticles, colloidal silver, wound covering, wound process