Immunotropic, antioxidant and reparative effects of a new thymogen analogue modified with D-alanine

Objective. The aim of the study was the investigation of the effects of a new Thymogen analogue modified with D-alanine from the C-terminus of the peptide and having the formula H₂N-L-Glu-L-Trp-D-Ala-COOH under conditions of skin wounds. Materials and methods. The peptide was administered once a day, parenterally (intraperitoneally) in an single dose of 1.2 μg / kg which is tenfold equimolar to Thymogen (1 μg / kg). The values of phagocytic and oxygen-dependent activities of blood neutrophils and levels of superoxide dismutase and malonic dialdehyde were detected. Also the histological investigation of skin wounds was carried out. Results. It has been established that an analogue of Thymogen does not impair the indicators of the absorption stage of blood neutrophils phagocytosis, the activity of superoxide dismutase and the number of lymphocytes in the skin in comparison with Thymogen and those are comparable to it. The Thymogen analogue, modified with D-alanine, has a more pronounced stimulation of oxygen-dependent neutrophil activity (NBT-test) and an increase in the number of fibroblasts in the skin, indicating a more pronounced immunotropic and reparative effects of this analogue. Conclusion. Thus, due to its introduction of the amino acid D-Ala (D-alanine) from the C-terminus into the Thymogen molecule , its stimulating effect on the oxygen-dependent function of neutrophils and wound-healing activity increases.

тимоген, аналог тимогена, кожные раны, функция нейтрофилов, антиоксидантная активность, репаративная регенерация кожи, Thymogen, Thymogen analogue, H₂N-L-Glu-L-Trp-D-Ala-COOH, skin wounds, the function of neutrophils, antioxidant activity, skin reparative regeneration