Comparative analysis of the effect of vitamin C and potassium orotate on the morphological picture during the implantation of a hernia mesh in an experiment

Viktor A. Lazarenko; Sergey V. Ivanov; Il’ya S. Ivanov; Aleksandr V. Ivanov; Andrey V. Tsukanov; Denis V. Tarabrin; Aleksey S. Kulabukhov; Ol’ga V. Tarabrina

doi:10.21626/vestnik/2019-1/04

Comparative analysis of the effect of vitamin C and potassium orotate on the morphological picture during the implantation of a hernia mesh in an experiment

Viktor A. Lazarenko, Sergey V. Ivanov, Il’ya S. Ivanov, Aleksandr V. Ivanov, Andrey V. Tsukanov, Denis V. Tarabrin, Aleksey S. Kulabukhov, Ol’ga V. Tarabrina

https://doi.org/10.21626/vestnik/2019-1/04

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Abstract

Objective. The aim of the research was a comparative experimental study of the effect of vitamin C and potassium orotate preparations on the formation of a connective tissue capsule around a mesh implant. Materials and methods. The experiment was performed on 150 white mice. The first group of animals received a standard diet after surgery. The diet of second and third animal groups was supplemented with vitamin C and potassium orotate, respectively. Histological sections obtained from the animals were placed on glass slides and stained with hematoxylin and eosin. The morphometry was performed afterwards in the capsule around the implant threads. Results. Being compared the cellular composition of the connective tissue regenerating around the hernia implant in the studied groups of animals showed that in both experimental groups, on the 7th day after implantation, fibroblastic cells predominated over non-resident cells. In the early postoperative period (7-30 days), the introduction of vitamin C into the diet contributed to a more rapid increase in the fibroblast population. A similar effect of potassium orotate is realized later, already at the stage of modeling and remodeling the capsule around the implant threads, on 60 and 90 days after the experimental surgical intervention. Conclusion. In the experiment, the effect of drugs in the zone of implantation of a hernioimplant was determined, which was manifested itself by a significantly more pronounced proliferation of fibroblast cells.