Peculiarities of determining 2-dimethylamine-1.3-bis- (phenylsulphonylthio) propane (bensultap) in vegetable raw materials

The advantages of isolating the analyzed compound from aboveground and underground parts of medicinal plants with a mixture of organic solvents acetonitrile-dichloroethane-ethyl acetate (6:2:2 by volume) are substantiated by double infusion with the duration of each infusion stage of 30 minutes and the mass ratio of the isolating agent to the biomatrix of 4:1. To purify 2-dimethylamino-1.3-bis-(phenylsulphonylthio) propane recovered from plant biomatrices, macrocolumnic adsorption chromatography on a 40/63 μ silica gel column and successive elution with hexane and a solvent system of diethyl ether-hexane (8:2 by volume) were used. A procedure was developed for the determination of 2-dimethylamino-1.3-bis (phenylsulphonylthio) propane in medicinal plant raw materials using the GC-MS method after preliminary thermal destruction of the test compound. The detection limit of 2-dimethylamino-1.3-bis-(phenylsulphonylthio) propane is 1.0∙10^-4 g in 100 g of biomaterial.

2-диметиламино-1, 3-бис-(фенилсульфонилтио)пропан (бенсултап), лекарственные растения, изолирование, очистка, ГХ-МС, определение, 2-dimethylamino-1.3-bis-(phenylsulphonylthio) propane (bensultap), medicinal plants, isolation, purification, GC-MS, determination

1. Баранов Ю.Н., Шорманов В.К., Коваленко Е.А. Методика определения 2-диметиламино-1,3-бис-(фенилсульфонилтио)пропана в крови и её валидация // Международный научно-исследовательский журнал. - 2018. - № 3 (69). - С. 88-94. [Baranov Yu.N., Shormanov V.K., Kovalenko E.A. Methodology of determination of 2-dimethylamino-1.3-bis- (phenylsulphonylthio) propane in blood and its validation. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal. 2018; 69(3): 88-94 (in Russ.)].

2. Григорьев А.М., Мельник А.А., Рудакова Л.В. Идентификация и определение производных и продуктов метаболизма банкола методами ГЖХ-МС и ВЭЖХ для целей судебно-химического и токсикологического анализа // Сорбционные и хроматографические процессы. - 2008. - Т. 8, вып. 5. - С. 766-778. [Grigor’yev A.M., Mel’nik A.A., Rudakova L.V. Identification and determination of derivatives and products of benzol metabolism by GLPC-MS and HPLC methods for the purposes of forensic and toxicological analysis. Sorbtsionnyye i khromatograficheskiye protsessy. 2008; 8(5): 766-778 (in Russ.)].

3. Григорьев А.М., Недовизина Г.В., Пирожков М.В. Определение производных и метаболитов бенсультапа (банкола) хроматографическими методами // Судебно-медицинская экспертиза. - 2009. - Т. 52, № 5. - С. 30-35. [Grigoriev A.M., Nedovizina G.V., Pirozhkov I.V. Detection of bancol derivatives and metabolites by chromatographic methods. Sudebno-meditsinskaya ekspertiza. 2009; 52 (5): 30-35 (in Russ.)].

4. Шорманов В.К., Баранов Ю.Н., Дурицын Е.П., Маслов С.В., Прониченко Е.И., Ганиев С.В. Судебно-химическое определение банкола // Судебно-медицинская экспертиза. - 2010. - Т. 53, № 6. - С. 39-41. [Shormanov V.K., Baranov Iu.N., Duritsyn E.P., Maslov S.V., Pronichenko E.I., Ganiev S.V. Detection of bancol for the purpose of forensic chemical expertise. Sudebno-meditsinskaya ekspertiza. 2010; 53 (6): 39-41 (in Russ.)].

5. Шорманов В.К., Баранов Ю.Н., Терских А.П., Неврова А.Ю., Щербакова М.Н. Определение бенсултапа в биологическом материале растительного происхождения // Курский научно-практический вестник «Человек и его здоровье». - 2014. - № 3. - С. 94-101. [Shormanov V.K., Baranov Yu.N., Terskikh A.P., Nevrova A.Yu., Shcherbakova M.N. Determination of bensultap in biological materials of vegetable origin. Kurskiy nauchno-prakticheskiy vestnik «Chelovek i ego zdorov’ye» - Kursk Scientific and Practical Bulletin "Man and His Health". 2014; (3): 94-101 (in Russ.)].

6. Bensultap [Электронный ресурс] // Lofter. - Режим доступа: http://docbook.blog.163.com/blog/static/208326047201262412227365/, свободный (20.01.2018). [Bensultap [Electronic resource]. Lofter. Access mode: http://docbook.blog.163.com/blog/static/208326047201262412227365/, free (01/20/2018)].

7. Bensultap [Электронный ресурс] // Pub Chem. - Режим доступа: https://pubchem.ncbi.nlm.nih.gov/compound/Bensultap#section=Top, свободный (20.01.2018). Bensultap [Electronic resource]. Pub Chem. Access mode: https://pubchem.ncbi.nlm.nih.gov/compound/Bensultap#section=Top, free (01/20/2018)].

8. Inoue M., Yamamoto A. An Analytical Method of Bensultap Residues in Crops and Soils // Journal of Pesticide Science. - 1986. - Vol. 11, N 4. - P. 547-555.

9. Kumar A.S., Amalnath D., Dutta T.K. Cartap poisoning: A rare case report // Indian J. Crit. Care Med. - 2011. - Vol. 15, N 4. - Р. 233-235. - DOI:10.4103/0972-5229.92075.

10. Kurisaki E., Kato N., Ishida T., Matsumoto A., Shinohara K., Hiraiwa K. Fatal human poisoning with Padan: a cartap-containing pesticide // Clin Toxicol (Phila). 2010. - Vol. 48, N 2. - P. 153-155 - DOI: 10.3109/15563650903505166.

11. Nguyen T.D., Lee K.J., Lee M.H., Lee G.H. A multiresidue method for the determination 234 pesticides in Korean herbs using gas chromatography mass spectrometry // Microchemical Journal. - 2010. - Vol. 95, N 1. - P. 43-49.

12. Núñez O., Gallart-Ayala H., Ferrer I., Moyano E., Galceran M.T. Strategies for the multi-residue analysis of 100 pesticides by liquid chromatography-triple quadrupole mass spectrometry // Journal of Chromatography A. - 2012. - Vol. 1249. - P. 164-180. - DOI: 10.1016/j.chroma.2012.06.028.

13. Rajković M.B., Perić L., Kovačević D. Quality of potatoes grown in various regions of Serbia as influenced by heavy metal and pesticide residues concentrations // Journal of Agricultural Sciences. - 2002. - Vol. 47, N 2. - P. 161-177.

14. Węgorek P. Current status of resistance incolorado potato beetle (leptinotarsa decemlineata say) to selected active substances of insecticides in Poland // Journal of Plant Protection Research. - 2005. - Vol. 45, N 4. - Р. 309-319.

15. Węgorek P., Zamojska J., Mrówczyński M. Susceptibility Level of the Colorado Potato Beetle (Leptinotarsa Decemlineata Say) to Chlorpyrifos and Acetamiprid in Poland and Resistance Mechanisms of the Pest to Chlorpyrifos // Journal of Plant Protection Research. - 2011. - Vol. 51, N 3. - Р. 279-284.