Development of a technique for the quantitative determination of the amount of flavonoids in the herb of Agastache rugosa
Evgeny E. Kurdyukov,
Ekaterina O. Garanina,
Alexander V. Mitishev,
Inessa Ya. Moiseeva,
Anna A. Mikurova https://doi.org/10.21626/vestnik/2026-2/10
EDN: UXTYRG
Abstract
Objective - to develop a methodology for the total content of flavonoids in the herb of Agastache rugosa by differential spectrophotometry. Materials and methods. The object of the study was dried grass Agastache rugosa, grown in the Penza region. Quantitative determination was performed by differential spectrophotometry at a wavelength of 385 nm using cinaroside as a standard. Results. Optimal conditions for flavonoid extraction have been established: 70% ethyl alcohol extractant, 1:50 ratio of "raw material - extractant", extraction time - 60 minutes, degree of grinding of raw materials - 1.0 mm. The amount of flavonoids in terms of cinaroside was 2.80±0.06%. A validation assessment of the methodology was carried out in terms of linearity, precision and accuracy; the relative error of a single determination with a confidence probability of 95% is ±2.68%. Conclusion. The developed technique can be used to standardize the herb mulberry as a promising source of flavonoids. This technique makes it possible to evaluate the quality of Agastache rugosa by the content of one of the main groups of active substances - flavonoids.
About the Authors
Evgeny E. Kurdyukov
Penza State University (PSU)
Russian Federation
Russian Federation
Ekaterina O. Garanina
Penza State University (PSU)
Russian Federation
Russian Federation
Alexander V. Mitishev
Penza State University (PSU)
Russian Federation
Russian Federation
Inessa Ya. Moiseeva
Penza State University (PSU)
Russian Federation
Russian Federation
Anna A. Mikurova
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Review
For citations:
Kurdyukov E.E., Garanina E.O., Mitishev A.V., Moiseeva I.Ya., Mikurova A.A. Development of a technique for the quantitative determination of the amount of flavonoids in the herb of Agastache rugosa. Humans and their health. 2026;29(2):74-80. (In Russ.) https://doi.org/10.21626/vestnik/2026-2/10. EDN: UXTYRG
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