A comparative study of hydroxyproline concentration in a periprosthetic capsule after subcutaneous placement of polymeric matrixes in an in vivo experiment

Objective - to evaluate the dynamics of hydroxyproline concentrations after subcutaneous placement of various polymeric matrices in an in vivo experiment, which would allow us to determine the characteristics of scar tissue formation during medical device implantation. Materials and methods. The study was conducted on 90 male Wistar rats, divided into three groups. The following samples were used: collagen matrix with glutaraldehyde (CGM), CGM with cultured dermal fibroblasts, and collagen wound healing dressing (CWHD). Implantation was performed into the subcutaneous tissue, with subsequent biopsy samples collected on days 7, 15, and 30. Hydroxyproline concentrations were determined colorimetrically, followed by statistical data processing. Results. The study showed different dynamics of hydroxyproline concentrations depending on the type of implanted material. In the early stages (7 days), the highest values were observed in the CGM with cells (0.562 mg/ml) and CGM (0.56 mg/ml) groups. By day 15, a decrease in the parameters was observed, and by day 30, a reverse trend was observed: in the SCM-cell group, the concentration was 0.129 mg/ml, in the SCM-cell group - 0.133 mg/ml, and in the SCM group - 0.2065 mg/ml. Conclusion. It was established that the matrix with cultured fibroblasts demonstrated the most favorable dynamics of periprosthetic capsule formation, characterized by a decrease in hydroxyproline concentration by the end of the experiment. These results demonstrate the potential of using cell-based technologies to optimize tissue regeneration processes during medical device implantation, which may find application in clinical practice for the prevention of excessive scarring.

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