Development of a technique for quantitative determination of procyanidins in shoots of Crataegus pinnatifida BUNGE using the Box-Behnken plan

The shoots of the hawthorn are a promising raw material of the Far Eastern phytocenoses, deserving the attention of domestic medicine. One of the classes of biologically active substances that ensure the pharmacotherapeutic activity of the plant are procyanidins.

Objective - development of a methodology for quantitative determination of the sum of procyanidins in shoots of hawthorn pistonadrass using the surface response methodology and the Box-Behnken plan.

Materials and methods. The shoots of the hawthorn were harvested in 2024 during the beginning phase of flowering in the natural population in Khabarovsk Territory. For quantitative determination of procyanidins, we used a modified Porter method based on acid hydrolysis of oligomeric procyanidins to anthocyanidins in the presence of a Fe³⁺ catalyst. The tests were performed on a UV-1700 spectrophotometer, Shimadzu (Japan). Statistical processing of the results was carried out in accordance with the GF XV edition of the OFS.1.1.0013 "Statistical processing of the results of physical, physico-chemical and chemical tests" using Microsoft Office Excel 2010 and Statistica 6.0 software packages.

Results. During the development of the technique, the conditions for the extraction of procyanidins from the shoots of hawthorn were optimized using the surface response methodology and the Box-Benken plan. It has been established that the maximum yield of procyanidins is achieved with the following combination of parameters: the extractant is ethyl alcohol 54%; the ratio of the mass of the raw material to the volume of the extractant is 1:116, the duration of extraction is 45 minutes. The single extraction mode is sufficient to yield a soluble fraction of procyanidins.

Conclusion. A technique has been developed for the quantitative spectrophotometric determination sum of procyanidins in the shoots of hawthorn, which will be used in solving a number of issues related to the standardization of Crataegus pinnatifida .

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