Study of the effect of the "Antiseptic Dorogov's stimulator" (ASD 2B-drops) on the growth of Mycobacterium tuberculosis strain

Sergey V. Engashev; Vladimir G. Kukes; Aleksandr V. Poddubikov; Nikita G. Sidorov; Anna Yu. Leonova; Olga K. Parfenova; Albina A. Gazdanova; Olga A. Dorogova

doi:10.21626/vestnik/2023-4/08

Study of the effect of the "Antiseptic Dorogov's stimulator" (ASD 2B-drops) on the growth of Mycobacterium tuberculosis strain

Sergey V. Engashev, Vladimir G. Kukes, Aleksandr V. Poddubikov, Nikita G. Sidorov, Anna Yu. Leonova, Olga K. Parfenova, Albina A. Gazdanova, Olga A. Dorogova

https://doi.org/10.21626/vestnik/2023-4/08

EDN: WLQSZD

Full Text:

PDF (Rus)

Generate QR code

Abstract

Objective - to study the effect of three different concentrations of the drug "ASD-2B drops" on the growth of M. tuberculosis strain № 22063. Materials and methods. The effect of three different concentrations of "ASD-2B drops" on the growth of M. tuberculosis strain № 22063 with a single addition of the drug into the reaction mixture was evaluated. Cultivation was carried out in Dubos broth for growing mycobacteria at 37°C for 20 days with periodic assessment of the turbidity index using a densitometer. Results. "ASD-2B drops" at a concentration of 750 µl in 10 ml of the reaction mixture after a single addition at the zero point inhibited the growth and accumulation of biomass of M.tuberculosis strain № 22063 for 10 days compared with the control. "ASD-2B drops" in lower concentrations (37.5 µl and 75 µl) contributed to an increase in the growth and accumulation of biomass of M.tuberculosis strain № 22063 compared with the control. Conclusion. The experiment demonstrated the ability of "ASD-2B drops" with a single addition to influence the growth and accumulation of biomass of M. tuberculosis strain № 22063, depending on the concentration of the drug. The presence of an inducing effect on the M. tuberculosis strain № 22063 provides a basis for further studies of the drug "ASD-2B drops".

Keywords

ASD-2B drops' M. tuberculosis, bacterial growth

About the Authors

Sergey V. Engashev

Agrovetzashchita LLC
Russian Federation

Dr. Sci. (Vet.), Professor, Academician of the Russian Academy of Sciences, General Director of Agrovetzashhita LLC, Moscow

Vladimir G. Kukes

Scientific Centre for Expert Evaluation of Medicinal Products (SCEEMP); I.M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation

Dr. Sci. (Med.), Academician of the Russian Academy of Sciences, Professor, RCEEMP, Moscow; Professor of the Department of Clinical Pharmacology and Propedeutics of Internal Diseases, Sechenov University, Moscow, Russian Federation.

Aleksandr V. Poddubikov

I.I. Mechnikov Research Institute of Vaccines and Sera (I.I. Mechnikov RIVS)
Russian Federation

Cand. Sci. (Med.), Head of the Laboratory of microbiology of opportunistic bacteria, I.I. Mechnikov RIVS, Moscow, Russian Federation

Nikita G. Sidorov

I.I. Mechnikov Research Institute of Vaccines and Sera (I.I. Mechnikov RIVS)
Russian Federation

Post-graduate student, junior researcher, Laboratory of microbiology of opportunistic bacteria, I.I. Mechnikov RIVS, Moscow, Russian Federation

Anna Yu. Leonova

I.I. Mechnikov Research Institute of Vaccines and Sera (I.I. Mechnikov RIVS)
Russian Federation

Junior researcher, Laboratory of microbiology of opportunistic bacteria, I.I.Mechnikov Research Institute of Vaccines and Sera, Moscow

Olga K. Parfenova

Institute of Biomedical Chemistry (IBMC)
Russian Federation

Junior researcher, Laboratory of Genetic Engineering and Methylomic Research, IBMC, Moscow, Russian Federation

Albina A. Gazdanova

I.M. Sechenov First Moscow State Medical University (Sechenov University)I.M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation

Cand. Sci. (Biol.), Associate Professor at the Department of Clinical Pharmacology and Propedeutics of Internal Diseases, Sechenov University, Moscow, Russian Federation

Olga A. Dorogova

Agrovetzashchita LLC
Russian Federation

Cand. Sci. (Med.), immunologist, scientific consultant of Agrovetzashchita LLC, Moscow, Russian Federation

References

1. Zaychikova M.V., Bespiatykh D.A., Malakhova M.V., Bodoev I.N., Vedekhina T.S., Veselovsky V.A., Klimina K.M., Varizhuk A.M., et al. Transcriptional profiling of Mycobacterium smegmatis exposed to subinhibitory concentrations of G4-stabilizing ligands. Bulletin of RSMU. 2022;(3):26-33. DOI: 10.24075/brsmu.2022.024

2. Korsunskiy I.A., Kudlay D.A. Diagnostics and control of cellular immunity of patients having tuberculosis. Pacific Medical Journal. 2021;(2):19-24 (in Russ.). DOI: 10.34215/1609-1175-2021-2-19-24. EDN: JRZDOX.

3. Sein O.B., Subbotina N.N. Immunobiological status in domestic animals when using a complex preparation obtained from biological waste. Vestnik Kurskoy gosudarstvennoy sel’skokhozyaystvennoy akademii. 2022;(4):75-81 (in Russ.).EDN: PUYLLR.

4. Makagon A.C., Red’kin S.V. Investigation of organoleptic and physico-chemical parameters of broiler chicken meat using ASD-2. Nauchnyy zhurnal. 2021;1(56): 36-38 (in Russ.). EDN: WKSALC.

5. Abdrakhmanov V.I., Krasnov V.L., Logutov V.I., Orlov A.V., Sakhipov V.R. Study of the chemical composition of the drug ASD-2F by chromatographic methods. Mezhdunarodnyy zhurnal prikladnykh i fundamental’nykh issledovaniy. 2019;(6):168-173 (in Russ). DOI: 10.17513/mjpfi.12788. EDN: JCXWSK.

6. Okolelova T.M., Engashev S.V., Salgereyev S.M., Lesnichenko I.Yu.Russian preparations for the production of environmentally friendly products. Effektivnoye zhivotnovodstvo. 018;3(142):46-48 (in Russ.). EDN: XORVFR.

7. Rodionov R.V., Krasnikova E.S., Belyakova A.S. Implication of new medicinal composition for the treatment of dyspepsia in calves from BLV-infected cows. Vestnik KrasGAU. 2019;2(143): 77-84. EDN: YZSYCD.

8. Malik E.V., Usha B.V., Rusanov I.A. Recovery colonizing resistance with diarrheal syndrome in newborn calves. Agrarian bulletin of the Urals. 2011;(12-1):8-9 (in Russ.). EDN: PAQSIH.

9. Okara A.I., Donets E.A., Savin I.M. Feed additives from non-traditional raw materials to increase poultry productivity. Myasnaya industriya. 2002;(10):40-42 (in Russ.)

10. Mannapova R.T., Rusanov I.A., Malik E.V., Usha B.V. Immunotsitologicheskie reaction in the bone marrow and their correction in the diarrhea syndrome in calves. Agrarian bulletin of the Urals. 2011;(7):19-20 (in Russ.). EDN: NNKNVI.

11. Moskalev V.G., Yengashev S.V., Engasheva E.S., Bogdanova N.E. Effectiveness of "ASIDIVIT" AND "ASD-2F" drugs in complex therapy of acute respiratory diseases of calves on industrial complexes. Vestnik Kurskoy gosudarstvennoy sel’skokhozyaystvennoy akademii. 2020;(2):22-27 (in Russ.). EDN: CFMMMZ.

12. Engashev S.V.1, Kukes V.G., Poddubikov A.V., Sidorov N.G., Parfenova O.K., Leonova A.YU., Dorogova O.A. Infectious diseases. 2021;19(3):104-108 (in Russ.). DOI: 10.20953/1729-9225-2021-3-104-108. EDN: MITXWS.

13. Engashev S.V., Dorogova O.A., Abramov V.E. Toxicological assessment of the drug ASD-2F. Akademicheskaya publitsistika. 2017;(2):286-306 (in Russ.). EDN: XXGZDT.

Review

For citations:

Engashev S.V., Kukes V.G., Poddubikov A.V., Sidorov N.G., Leonova A.Yu., Parfenova O.K., Gazdanova A.A., Dorogova O.A. Study of the effect of the "Antiseptic Dorogov's stimulator" (ASD 2B-drops) on the growth of Mycobacterium tuberculosis strain. Humans and their health. 2023;26(4):88-92. (In Russ.) https://doi.org/10.21626/vestnik/2023-4/08. EDN: WLQSZD

This work is licensed under a Creative Commons Attribution 4.0 License.

ISSN 1998-5746 (Print)
ISSN 1998-5754 (Online)

Username
Password
	Remember me
Not a user? Register with this site Forgot your password?

User

Humans and their health

Study of the effect of the "Antiseptic Dorogov's stimulator" (ASD 2B-drops) on the growth of Mycobacterium tuberculosis strain

Full Text:

Abstract

Keywords

About the Authors

References

Review

For citations:

Cookies policy