Study of polysaccharides of common raspberry shoots and their sorption activity

A comparative study of the water-soluble polysaccharide complex and pectin substances of first and second year shoots of common raspberry. Objective - comparative study and sorption activity of polysaccharides of common raspberry shoots. Materials and methods. The samples for the research were collected in a dark coniferous forest on the territory of the Ilyinsky district of Perm Region. The content of polysaccharide fractions was determined by the modified Dreywood anthrone- sulfurous method. A water-soluble polysaccharide complex and pectin substances were sequentially obtained from different parts of the shoots. The sorption activity was determined by the ability of the substances to bind methylene blue, which is a marker for most medical sorbents. The acute toxicity of the obtained polysaccharide fractions was examined according to Prozorovsky V.V. Results. The shoots of the first and second year of common raspberry accumulate more pectin substances than water-soluble polysaccharides. Leaves of raspberry contain more polysaccharides compared to leafless parts. As a result of acute toxicity study, it was found that all studied substances can be classified as low-toxic (LD50>5,000 mg/kg). It was found that water-soluble polysaccharide complex obtained from leafless parts of the shoot of the first and second year shows sorption activity reliably higher than that of the reference preparation (Polyphepan). Sorption activity of pectin substances obtained from leaves of the first and second year shoots also exceeds that of the reference preparation. The highest sorption activity is shown by pectin substances of the second year, and the lowest sorption activity by pectin substances of leafless raspberry shoots. Conclusion. The obtained substances are promising for creating modern sorbents based on polysaccharide fractions of the first and second year shoots of common raspberry. Taking into account the simplicity in harvesting and high sorption activity of polysaccharides of the leafless part of the raspberry shoot, not only leaves but also leafless parts of the shoot should be harvested.

1. Bystrushkin A.G. On the life form of the common raspberry (Rubus idaeus L.). Vestnik Cheljabinskogo gosudarstvennogo universiteta. 2005;12(1):52-55 (in Russ.). EDN: NTZCWN

2. Committee on Herbal Medicinal Products (HMPC).Community Herbal Monograph on Rubus idaeus L. Folium. London: European Medicines Agency, 2012. 22 p.

3. Zavrazhnov V.I., Kitayeva R.I., Khmelev K.F. Medicinal plants. Therapeutic and preventive use. Voronezh: Voronezh University Press, 1993. 480 p. (in Russ.)

4. Hummer K.E.Rubus pharmacology: antiquity to the present. Horticultural Science. 2010;45(11): 1587-1591

5. Sheng J.Y., Wang S.Q., Liu K.H., Zhu B., Zhang Q.Y., Qin L.P., Wu J.J.Rubus chingii Hu: an overview of botany, traditional uses, phytochemistry, and pharmacology. Chinese Journal of Natural Medicines. 2020;18(6):401-416. DOI: 10.1016/S1875-5364(20)30048-0

6. Niu, Y., Wang, H., Xie, Z., Whent, M., Gao, X., Zhang, X., Zou, S., Yao, W. et al. Structural analysis and bioactivity of a polysaccharide from the roots of Astragalus membranaceus (Fisch) Bge. var. mongolicus (Bge.) Hsiao. Food Chemistry. 2011;128(3):620-626. DOI: 10.1016/J.FOODCHEM.2011.03.055

7. Yu Z., Liu L., Xu Y., Wang L., Teng X., Li X., Dai J. Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits. Carbohydr Polym. 2015;132:180-186. DOI: 10.1016/j.carbpol.2015.06.068

8. Ren-Bo X., Xin Y., Jing W., Hai-Tian Zh., Wei-Hong L., Jie C., Cui-Lin Ch., Pan Z., Xu R.B., Yang X., Wang J., Zhao H.T., Lu W.H., Cui J., Cheng C.L., Zou P. et al. Chemical composition and antioxidant activities of three polysaccharide fractions from pine cones.Int J Mol Sci. 2012;13(11):14262-14277. DOI: 10.3390/ijms131114262

9. Zaid R.M., Mishra P., Wahid Z.A., Sakinah A.M.M. Hylocereus polyrhizus peel's high-methoxyl pectin: A potential source of hypolipidemic agent.Int J Biol Macromol. 2019;134:361-367. DOI: 10.1016/j.ijbiomac.2019.03.143

10. Chen R., Liu Z., Zhao J., Chen R., Meng F., Zhang M., Ge W. Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu. Food Chem. 2011;127(2):434-440. DOI: 10.1016/j.foodchem.2010.12.143

11. Rakhmanberdyeva R.K., Shashkov A.S., Bobakulov K.M., Azizov D.Z., Malikova M.K., Ogay D.K. The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica. Carbohydr Res. 2021;505:108342. DOI: 10.1016/j.carres.2021.108342

12. Du Y., Wan H., Huang P., Yang J., He Y. A critical review of Astragalus polysaccharides: From therapeutic mechanisms to pharmaceutics. Biomed Pharmacother. 2022;147:112654. DOI: 10.1016/j.biopha.2022.112654

13. Yin M., Zhang Y., Li H. Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides. Front Immunol. 2019;10:145. DOI: 10.3389/fimmu.2019.00145

14. Xie J.H., Jin M.L., Morris G.A., Zha X.Q., Chen H.Q., Yi Y., Li J.E., Wang Z.J. et al. Advances on Bioactive Polysaccharides from Medicinal Plants. Crit Rev Food Sci Nutr. 2016;56 Suppl 1:S60-84. DOI: 10.1080/10408398.2015.1069255

15. Liu J., Willfоr S., Xu C. A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications. Bioactive Carbohydrates and Dietary Fibre. 2015;5(1):31-61. DOI: 10.1016/j.bcdf.2014.12.001

16. de Souza P., Boeing T., Somensi L.B., Cechinel-Zanchett C.C., Bastos J.K., Petreanu M., Niero R., Cechinel-Filho V. et al. Diuretic effect of extracts, fractions and two compounds 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid and 5-hydroxy-3,6,7,8,4'-pentamethoxyflavone from Rubus rosaefolius Sm. (Rosaceae) leaves in rats. Naunyn Schmiedebergs Arch Pharmacol. 2017;390(4):351-360. DOI: 10.1007/s00210-016-1333-4

17. Rojas-Vera J., Patel A.V., Dacke C.G. Relaxant activity of raspberry (Rubus idaeus) leaf extract in guinea-pig ileum in vitro. Phytother Res. 2002;16(7):665-668. DOI: 10.1002/ptr.1040

18. Ma H., Johnson S.L., Liu W., DaSilva N.A., Meschwitz S., Dain J.A., Seeram NP. Evaluation of Polyphenol Anthocyanin-Enriched Extracts of Blackberry, Black Raspberry, Blueberry, Cranberry, Red Raspberry, and Strawberry for Free Radical Scavenging, Reactive Carbonyl Species Trapping, Anti-Glycation, Anti-β-Amyloid Aggregation, and Microglial Neuroprotective Effects.Int J Mol Sci. 2018;19(2):461. DOI: 10.3390/ijms19020461

19. Seeram N.P., Adams L.S., Zhang Y., Lee R., Sand D., Scheuller H.S., Heber D. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J Agric Food Chem. 2006;54(25):9329-9339. DOI: 10.1021/jf061750g

20. Gulyayev D.K., Belonogova V.D., Rudakova I.P.Composition and biological activity of polysaccharides of shoots and leaves of raspberries (Rubus idaeus). Traditional medicine. 2017;4(51):39-42 (in Russ.). EDN: YNJTAA

21. Krauze-Baranowska M., Głód D., Kula M., Majdan M., Hałasa R., Matkowski A., Kozłowska W., Kawiak A. Chemical composition and biological activity of Rubus idaeus shoots--a traditional herbal remedy of Eastern Europe. BMC Complement Altern Med. 2014;14:480. DOI: 10.1186/1472-6882-14-480

22. Bailey R.W., Haq S., Hassid W.Z. Carbohydrate composition of particulate preparations from mung bean (Phaseolus aureus) shoots. Phytochemistry. 1967;6(2):293-301

23. Olennikov D.N., Tankhayeva L.M. Method of quantitative determination of the group composition of the carbohydrate complex of plant objects. Khimija Rastitel’nogo Syr’ja. 2006;(4):29-33 (in Russ.). EDN: HYINOX

24. Prozorovskiy V.V., Prozorovskaya V.M. Express method for determining the average lethal dose. Farmakologiya i toksikologiya. 1978;(4):497-502 (in Russ.)

25. Reshetnikov V.I. Principles of development of medicinal forms of sorbents. Perm: GOU VPO PGFA Roszdrava, 2008. 196 p. (in Russ.)

26. Velichko V.V., Makarova D.L.Comparative pharmacognostic analysis of leaves and fruit of raspberry ordinary. Medicina i obrazovanie v Sibiri. 2015;(4):16-24. (in Russ.). EDN: UXTHXR