Morphological features of the rats’ large intestine with stress-induced dysbiosis

Abstract

Objective. The aim was to study the morphological features of the rats’ colon with stress-induced dysbiosis. Materials and methods. The work was performed on 26 Wistar male rats, which were divided into two groups: the first group was intact (control) one; the animals of the second group were under conditions of chronic restraint stress in the form of movement restriction by confining in individual plastic boxes for 2 hours daily for 14 days. After this period, the animals were taken out of the experiment, followed by sampling the colon, which was examined histologically and morphometrically. Results. Under stress, changes of an acute inflammatory nature developed, phenomena of surface architectonics reorganization were noted. The number of goblet cells was reduced; the cell composition was predominantly represented by lymphocytes, plasmocytes and macrophages, single neutrophils. A sharp increase in the number of mast cells was recorded as compared to the control, most of them being in degranulated form (type 4). The data obtained allow us to note the restructuring of the histological structures of the colon of experimental animals under the influence of chronic restraint stress and are consistent with modern ideas about the pro-inflammatory effect of stress on intestines. Conclusion. Under conditions of chronic restraint stress in the rats’ colon, the formation of dysbiosis occurs against the background of the morphological changes of inflammatory and destructive nature in its wall.