Activity of matrix metalloproteinases 1, 9, 19 tissue inhibitor 1 in the structural components of a nephron in rats with a destructive-inflammatory process in the urinary system

Morphological components of the nephron perform different functions in the urinary system. Each structural component includes a matrix and enzymes that regulate its remodeling in normal and pathological conditions. Objective - to determine the activity of metalloproteinases -1, -9, -19, their tissue inhibitor 1 in the structures of the vascular glomerulus, proximal, distal tubules, tubule loop, collecting tubule of the nephron in rats with experimental ascending kidney infection. Materials and methods. In an experiment on 16 Wistar female rats (since the female sex develops an inflammatory process in the kidneys more often that men) under rometar and lidocaine (according to the instructions), modeled a control series and an ascending kidneys infection. In this case 0,1ml of fecal suspension was inoculated unto the bladder with an insulin suringe by injection , obtained at the rate of 50 mg per 1 ml of salin solution with triple filtration through 3 lays of layers of gauze. The assessment and standardization of microbial contamination of inoculums by obligate representatives of the intestinal microbiota was perfomed by seeding a prepared suspension of feces on an Endo medium and yolk-salt agar-ZHSA with subsequent counting after 1-2 days, the purity of the grown colonies. When 0.1 ml of suspension is injected into the bladder, microbes of the Enterobacteria group is 27111.1±1911.6, Staphylococcus is 8333±1632.9. After 31 days from the moment of modeling, the kidneys were taken away. The activity of metalloproteinases (MMPs) was evaluated by immunohistochemical method by determining the number of antigen-positive cells to MMPs and the intensity of their expression. Results. The study showed that after 31 days from the moment of modeling the destructive-inflammatory process in the system of urination in the vascular glomerulus, the loop of tubules, the activity of the studied MMP and TIMP increased. In the proximal and distal tubules, MMP 1 and TIMP 1 decreased against the background of increased activity of MMP 19. At the same time, the activity of MMP 1, MMP 19 and TIMP 1 increased in the collecting tubes. Conclusion. A comparative study showed a significant shift in the activity of the studied metalloproteinases and their tissue inhibitor from the cortical substance, where the vascular glomeruli, proximal and distal tubules are located, to the medulla - the zone of concentration of the loops of tubules and collecting tubules.

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