Analysis of the amino acid and elemental composition of white mistletoe leaves

Abstract

Objective: comparative study of the amino acid and elemental composition of leaves Viscum album L. and host plants. Materials and methods. The amino acid and elemental composition in raw materials and extracts from leaves Viscum album and the corresponding host plant, obtained by extraction with 50% ethyl alcohol, was determined by capillary electrophoresis. Results. Glycine, alanine, leucine, proline, methionine, valine, asparagine, aspartic and glutamic acids were previously identified in the leaves Viscum album by ascending paper chromatography. By capillary electrophoresis, it was found that the raw materials and extracts contained proline, valine, alanine, threonine and methionine in the greatest amount. It has been established that in a semi-parasitic plant, the content of proline is 16 times higher than in Malus domestica Borkh. leaves and 5 times higher than in Pyrus communis L. leaves In alcohol-water extracts, the proline content is 5 and 1,5 times higher, respectively. It should be noted that in raw materials and extracts from Viscum album, obtained by extraction with 50% ethyl alcohol, potassium ions predominates quantitatively. Compared to leaves Malus domestica, leaves Viscum album accumulate 4,5 times more potassium, and 1,5 times more than in Pyrus communis leaves; in extracts, the content is also higher by 3 and 2 times, respectively. Conclusion. In raw materials and extracts from Viscum album, 13 free amino acids were found, of which 6 are essential (Arg, Phe, Leu, Met, Thr, Val). It was found that potassium predominates quantitatively in raw materials and extracts from Viscum album.