kurskvestЧеловек и его здоровьеHumans and their health1998-57461998-5754Kursk State Medical University10.21626/vestnik/2021-2/05kurskvest-919Research ArticleМЕДИКО-БИОЛОГИЧЕСКИЕ НАУКИMEDICOBIOLOGICAL SCIENCESАнализ ретинальной экспрессии генов, участвующих в регуляции ангиогенеза и функций эндотелиального барьера после воздействия лазерного излучения с длиной волны 577 нм в непрерывном режиме на сетчаткуAnalysis of retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function following exposure of the retina to 577 nm wavelength laser light in continuous modehttps://orcid.org/0000-0003-0368-296XГавриловаНаталья АлександровнаGavrilovaNatalia A.

д-р мед. наук, профессор, зав. кафедрой глазных болезней

Dr. Sci. (Med.), Professor, Head of the Department of Eye Diseases

n.gavrilova@mail.ruhttps://orcid.org/0000-0002-7603-6064ГуревичКонстантин ГеоргиевичGurevichKonstantin G.

д-р мед. наук, профессор, зав. кафедрой ЮНЕСКО «Здоровый образ жизни - залог успешного развития»

Dr. Sci. (Med.), Professor, Head of the Department of the UNESCO Chair "Healthy lifestyle - the key to successful development"

kgurevich@mail.ruhttps://orcid.org/0000-0002-7337-0833КомоваОльга ЮрьевнаKomovaOlga Yu.

ассистент кафедры глазных болезней

Assistant Lecturer of the Department of Eye Diseases

ol-komo@yandex.ruhttps://orcid.org/0000-0003-4373-3135ЗиновьеваАлександра ВитальевнаZinovevaAlexandra V.

старший лаборант кафедры глазных болезней

Senior Laboratory Assistant of the Department of Eye Diseases

aleksandra.r@live.ruМосковский государственный медико-стоматологический университет имени А.И. ЕвдокимоваРоссияA.I. Yevdokimov Moscow State University of Medicine and DentistryRussian Federation2021231020212423745Copyright © Гаврилова Н.А., Гуревич К.Г., Комова О.Ю., Зиновьева А.В., 20212021Гаврилова Н.А., Гуревич К.Г., Комова О.Ю., Зиновьева А.В.Gavrilova N.A., Gurevich K.G., Komova O.Y., Zinoveva A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.kursk-vestnik.ru/jour/article/view/919

Цель исследования - изучение ретинальной экспрессии генов, участвующих в регуляции ангиогенеза и функций эндотелиального барьера после воздействия лазерного излучения с длиной волны 577 нм в непрерывном режиме на сетчатку в экспериментальных условиях после интравитреального введения VEGF165 (Vascular endothelial growth factor).

Материалы и методы

Материалы и методы. Исследование проводилось на 4-5-недельных самцах мышей линии C57BL/6J. Было сформировано 4 группы по 5 мышей в каждой группе, один глаз животных был экспериментальным, второй глаз оставался неповрежденным. Животным первой группы проводили интравитреальную инъекцию фосфатно-солевого буфера (PBS); животным второй, третьей и четвертой групп проводили интравитреальную инъекцию 50 нг/мл рекомбинантного VEGF165; в третьей и четвертой группах через один день после интравитреального введения VEGF165 производилось лазерное воздействие с длиной волны 577 нм на сетчатку в микроимпульсном и непрерывном режимах соответственно. Образцы тканей у животных из первой и второй групп были взяты через 2 дня после введения PBS и VEGF165, у животных из третьей и четвертой групп через один день после лазерного воздействия на сетчатку.

Результаты

Результаты. Изучена ретинальная экспрессия генов, участвующих в регуляции ангиогенеза и функций эндотелиального барьера в результате воздействия на сетчатку лазерного излучения 577 нм в непрерывном режиме после интравитреального введения VEGF165. Идентифицированы гены с достоверным изменением уровней экспрессии.

Заключение

Заключение. Понимание механизмов модуляции экспрессии генов сетчатки может помочь выявить ключевые регуляторные факторы, обеспечивающие терапевтический эффект лазерного излучения в непрерывном и микроимпульсном режимах, а также послужить основой для реализации будущей терапевтической тактики лечения заболеваний сетчатки.

Objective

Objective. The aim of the study was to investigate the retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function after exposure of the retina to 577 nm laser light in a continuous mode in experimental conditions after intravitreal injection of VEGF165 (Vascular endothelial growth factor).

Materials and methods

Materials and methods. The study was performed on 4-5 week old male C57BL / 6J mice. 4 groups, 5 mice in each, were formed, one animal eye was experimental and the second remained intact. The animals of the first group received intravitreal injection of phosphate-buffered saline (PBS); animals in the second, third and fourth groups received intravitreal injection of 50 ng/ml of recombinant VEGF165; in the third and fourth groups, one day after the VEGF165 intravitreal administration, laser irradiation with a wavelength of 577 nm was performed on the retina in micropulse and continuous modes, respectively. Tissue samples from animals of the first and second groups were taken 2 days after PBS and VEGF165 administration, and in animals from the third and fourth groups one day after laser exposure to the retina.

Results

Results. Retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function as a result of retinal exposure to 577 nm laser radiation in continuous mode following intravitreal injection of VEGF165 was studied. Genes with significant changes in expression levels were identified (genes that regulate the processes of angiogenesis).

Conclusion

Conclusion. Understanding the mechanisms of modulation of retinal gene expression may help to identify the key regulatory factors providing therapeutic effects of laser radiation in continuous and micropulsed modes and provide the basis for future therapeutic tactics for retinal diseases.

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The authors declare that there are no conflicts of interest present.