kurskvestЧеловек и его здоровьеHumans and their health1998-57461998-5754Kursk State Medical University10.21626/vestnik/2024-3/10616.892.32: 576.311.347kurskvest-1342Research ArticleМЕДИКО-БИОЛОГИЧЕСКИЕ НАУКИMEDICOBIOLOGICAL SCIENCESИзменение реакций митохондриального биогенеза и митофагии в тканях коры больших полушарий и гиппокампа у крыс Wistar в условиях экспериментальной болезни АльцгеймераChanges in the reaction of mitochondrial biogenesis and mitophagy in the tissues of the cerebral cortex and hippocampus in Wistar rats under conditions of experimental Alzheimer's diseasehttps://orcid.org/0000-0002-5595-8182ПоздняковДмитрий ИгоревичPozdnyakovDmitry I.

канд. фарм. наук, доцент, зав. кафедрой фармакологии с курсом клинической фармакологии, ПМФИ - филиал ВолгГМУ, г. Пятигорск

Cand. Sci. (Pharm.), Associate Professor, Head of the Department of Pharmacology with a course in Clinical Pharmacology, PMPI - branch of VolgSMU, Pyatigorsk, Russian Federation

pozdniackow.dmitry@yandex.ruhttps://orcid.org/0009-0009-9892-0326ВихорьАнастасия АлексеевнаVikhor’Anastasia A.

студент, ПМФИ - филиал ВолгГМУ, г. Пятигорск

Student, PMPI - branch of VolgSMU, Pyatigorsk, Russian Federation

nastyavichori@gmail.comПятигорский медико-фармацевтический институт - филиал Волгоградского государственного медицинского университета (ПМФИ - филиал ВолгГМУ)РоссияPyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd state Medical University (PMPI - branch of VolgSMU)Russian Federation2024291120242739297Copyright © Поздняков Д.И., Вихорь А.А., 20242024Поздняков Д.И., Вихорь А.А.Pozdnyakov D.I., Vikhor’ A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.kursk-vestnik.ru/jour/article/view/1342

Цель - изучить изменение процессов митохондриального биогенеза и митофагии в коре больших полушарий и гиппокампе у крыс Wistar обоего пола в условиях экспериментальной болезни Альцгеймера. Материалы и методы. Болезнь Альцгеймера воспроизводили у самцов и самок крыс Wistar путем инъекции агрегатов β-амилоида 1-42 в СА1 часть гиппокампа. После 30 дней экспозиции крыс декапитировали, извлекали головной мозг, выделяли гиппокамп и кору больших полушарий с дальнейшим получением митохондриальной фракции, в которой оценивали изменение активности сукцинатдегидрогеназы, цитохром-с-оксидазы (биомаркеров митохондриального биогенеза) и цитратсинтазы (биомаркера митофагии) спектрофотометрическим методом. Результаты. Установлено, что у крыс Wistar обоего пола в условиях экспериментальной болезни Альцгеймера по сравнению с ложнооперированными животными отмечается снижение активности сукцинатдегидрогеназы, цитохром-с-оксидазы и цитратсинтазы как в коре больших полушарий, так и в гиппокампе. При этом пол-ассоциированных отличий активности изучаемых ферментов в гиппокампе не зафиксировано, тогда как в коре больших полушарий активность сукцинатдегидрогеназы и цитохром-с-оксидазы у крыс самок была ниже, чем у самцов, на 23,4% (p<0,05) и 25,3% (p<0,05) соответственно. Заключение. Проведенное исследование показало, что у крыс Wistar отмечаются пол-ассоциированные отличия реакций митохондриального биогенеза в коре больших полушарий, в то время как в гиппокампе достоверных отличий в реакциях митофагии и биогенеза митохондрий у крыс самок и самцов Wistar не установлено.

Objective - to evaluate changes in the processes of mitochondrial biogenesis and mitophagy in the cerebral cortex and hippocampus in Wistar rats of both genders in experimental Alzheimer's disease. Materials and methods. Alzheimer's disease was modeled in male and female Wistar rats by injection of β-amyloid aggregates 1-42 into the CA1 hippocampal part. After 30 days of exposure, the rats were decapitated, the brain was extracted, the hippocampus and cerebral cortex were isolated with further reception of the mitochondrial fraction, in which changes in the activity of succinate dehydrogenase, cytochrome c oxidase (biomarkers of mitochondrial biogenesis) and citrate synthase (biomarker of mitophagy) were evaluated by spectrophotometric method. Results. It was found that in Wistar rats of both genders under conditions of experimental Alzheimer's disease, compared to sham operated animals, there is a decrease in the activity of succinate dehydrogenase, cytochrome c oxidase and citrate synthase, both in the cerebral cortex and in the hippocampus were observed. At the same time, there were no gender differences in the activity of the studied enzymes in the hippocampus, whereas in the cerebral cortex, the activity of succinate dehydrogenase and cytochrome c oxidase in female rats was lower than in males by 23.4% (p<0.05) and 25.3% (p<0.05), respectively. Conclusion. The study showed that in Wistar rats there are gender differences in the reactions of mitochondrial biogenesis in the cerebral cortex, while in the hippocampus there are no significant differences in the reactions of mitophagy and mitochondrial biogenesis in female and male Wistar rats.

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The authors declare that there are no conflicts of interest present.